Search results for "dynamic nuclear"
showing 6 items of 6 documents
Drug Screening Boosted by Hyperpolarized Long-Lived States in NMR
2014
International audience; : Transverse and longitudinal relaxation times (T1ρ and T1 ) have been widely exploited in NMR to probe the binding of ligands and putative drugs to target proteins. We have shown recently that long-lived states (LLS) can be more sensitive to ligand binding. LLS can be excited if the ligand comprises at least two coupled spins. Herein we broaden the scope of ligand screening by LLS to arbitrary ligands by covalent attachment of a functional group, which comprises a pair of coupled protons that are isolated from neighboring magnetic nuclei. The resulting functionalized ligands have longitudinal relaxation times T1 ((1) H) that are sufficiently long to allow the powerf…
Dynamic nuclear polarisation of biological matter
1986
Polarised targets as used in high energy physics experiments may be of considerable interest in biological structure research using polarized neutrons. So far, this promising method has been facing difficulties in getting reasonable polarization of the target nuclei. We report on a polarized "frozen spin" target which has been prepared from an enzyme dissolved in a mixture of heavy water and deuterated propanediol doped with a completely deuterated paramagnetic radical. Clusters of 700 protons defined by the structure of lysozyme embedded in a fully deuterated matrix were polarized to 75% within an hour by 4 mm microwave irradiation in a magnetic field of 2.5 tesla at a temperature of 0.3 K…
Measurement of proton and nitrogen polarization in ammonia and a test of equal spin temperature
1998
The 1996 data taking of the SMC experiment used polarized protons to measure the spin-dependent structure function g(1) of the proton. Three liters of solid granular ammonia were irradiated at the Bonn electron linac in order to create the paramagnetic radicals which are needed for polarizing the protons. Proton polarizations of +/- (90 +/- 2.5)% were routinely reached. An analysis based on a theoretical line shape for spin-1. systems with large quadrupolar broadening was developed which allowed the nitrogen polarization in the ammonia to be determined with a 10% relative error. The measured quadrupolar coupling constant of N-14 agrees well with earlier extrapolated values. The polarization…
The polarized double cell target of the SMC
1999
The polarized target of the Spin Muon Collaboration at CERN was used for deep inelastic muon scattering experiments during 1993-1996 with a polarized muon beam to investigate the spin structure of the nucleon. Most of the experiments were carried out with longitudinal target polarization and 190 GeV muons, and some were done with transverse polarization and 100 GeV muons. Protons as well as deuterons were polarized by dynamic nuclear polarization (DNP) in three kinds of solid materials - butanol, ammonia, and deuterated butanol - with maximum degrees of polarization of 94%, 91% and 60%, respectively. Considerable attention was paid to the accuracies of the NMR polarization measurements and …
A Ramsey apparatus for the measurement of the incoherent neutron scattering length of the deuteron
2008
Abstract A Ramsey apparatus for cold, polarised neutrons is described, which enables us to measure neutron precession angles with an absolute accuracy of about 1 ∘ . This is necessary to perform a planned high-accuracy measurement of the incoherent neutron scattering length b i , d of the deuteron. The performance of the apparatus is demonstrated in systematic stability measurements as well as in two selected examples using samples containing polarised nuclear spins.
Resonant X-ray scattering in biological structure research
2007
The use of anomalous X-ray scattering of light elements like sulfur and phosphorus is of particular interest in biological structure research. These elements serve as native labels in proteins, nucleic acids and membranes. Their medium scattering power is drastically changed at their K absorption edges at wavelengths between 5 and 6 A where X-ray absorption excludes the use of open air diffractometers. The construction of a new diffractometer tunable to wavelengths between 1.2 and 7 A is presented. First results of anomalous scattering from sulfur in bacteriorhodopsin near the K absorption edge have been obtained recently. Their possible impact on crystallography will be considered. A compa…